AFFINE way to visualize the interaction between the bacterial cell or fragments thereof and affine surface using Atomic Force Microscopy

摘要

FIELD: medicine.;SUBSTANCE: method involves specific immobilisation of fragments of the specified bacteria from a solution on an affine surface consisting of a protein G layer coated with a layer of antibodies specific to the identified bacterial cells. Two-stage washing of a sample from a non-specifically bound material implies washing in a buffer pH 8.5-9.5 and washing in deionised water. The atomic-force microscopy is used as a visualisation tool of the bound bacterial fragments and enables direct count of a bound analyte amount per a surface unit.;EFFECT: offered invention allows identifying the minute cell concentration in the analysed solution.;1 dwg