METHOD OF MODELLING ENDOTHELIAL DYSFUNCTION BY REPRODUCTION OF METABOLIC DISORDERS

摘要

FIELD: medicine.;SUBSTANCE: invention relates to medicine, in particular, to experimental cardiopharmacology, and can be used for modelling and correction of endothelial dysfunction. For this purpose in experiment endothelial dysfunction is modelled by creation of hypoestrogenic condition by bilateral ovariectomy in laboratory animal and during 6 weeks by addition into food of fructose, specific weight of which constitutes 60% of total ration, as well as by daily single irritation by electric current with 10 ms, voltage 30 V, intensity 0.66 mA, passed through electrodes, applied on animal's tail moistened with physiological solution. Degree of endothelial dysfunction development is evaluated by ratio of endothelium-independent and endothelium-dependent vasodilation, and formation of metabolic disorders by change of glycemia level by 120 minute in oral test on tolerance to glucose. Claimed model can be applied for estimation of pharmacological correction of endothelial dysfunction in reproduction of metabolic disorders.;EFFECT: method makes it possible to estimate dynamics of formation of endothelial dysfunction in modelling of metabolic disorders with application of fructose-containing diet and electric current.;1 ex, 3 tbl, 1 dwg