METHOD AND SET FOR ELISA DETERMINATION OF FUNCTIONAL ACTIVITY OF HUMAN COMPLEMENT COMPONENT C1q

摘要

FIELD: medicine.;SUBSTANCE: sorption of pharmacy drug derinate, representing sodium deoxyribonucleate in micropanel holes is carried out. After that analysed sample, which contains component C1q with unknown activity, is introduced into holes. Incubation is carried out and after washing and drying of panel into holes introduced are conjugate of enzyme with antibodies against component C1q and substrate of said enzyme. Activity of component C1q is calculated by amount of formed product of enzymatic reaction. Set contains flat-bottom micropanel with sorbed derinate, conjugate of enzyme with antibodies to human complement component C1q, substrate buffer and standard with known C1q activity.;EFFECT: method application makes it possible to increase reliability of determination C1q component with application as activator of available and stable preparation derinate.;2 cl, 1 dwg, 2 ex