METHOD AND SET FOR IMMUNE-ENZYME ASSESSMENT OF FUNCTIONAL BYPASS ACTIVITY OF HUMAN COMPLEMENT COMPONENT C9

摘要

FIELD: medicine.;SUBSTANCE: there are offered a method and a kit for assessment of functional bypass activity of human complement component C9. The pharmaceutical preparation lisocyme is sorbed in microplate wells; an analysed sample containing a human complement component C9 with unknown activity and a porpoise complement preparation are introduced and incubated. After the plate is washed out and dried, conjugated enzyme and human complement component C9 antibodies and a substratum of this enzyme are introduced in the wells. The component C9 activity is evaluated by the amount of the prepared product of enzymatic reaction. The kit comprises the flat-bottomed microplate with sorbed lisocyme, conjugated enzyme and human complement component C9 antibodies, porpoise complement, substrate buffer and standard with known activity C9.;EFFECT: invention allows assessing functional activity of human complement component C9 by means of the new complement bypass activator - lisocyme.;2 cl, 1 dwg, 2 ex