METHOD OF PLACENTAL HOMOGENATE ANALYSIS FOR HEPARIN CONCENTRATION IN PUERPERANTS UNDERWENT ACUTE HERPES VIRAL INFECTION

摘要

FIELD: medicine.;SUBSTANCE: invention describes a method of evaluating the heparin concentration in puerperants underwent acute herpes viral infection in the third trimester, characterised by the fact that a piece of placenta is sampled from the puerperants, homogenate is prepared and processed for glycosaminoglycans recovery. Thereafter, the prepared glycosaminoglycans extracts are separated by gel electrophoresis in a polyacrylamide gel, and a heparin percentage is calculated by optical density sensitometry; a herpes viral infective episode is stated by spectrophotometry and shown by increasing titre of herpes virus antibodies.;EFFECT: higher analysis accuracy.;1 ex