Hikarigoke culture method

摘要

PROBLEM TO BE SOLVED: To provide a simple method for culturing Schistostega osmundacea, by which Schistostega osmundacea protonemata weak to change in environment can be efficiently proliferated in a large amount.;SOLUTION: This method for culturing the Schistostega osmundacea includes a protonema proliferation process for culturing Schistostega osmundacea gametophore washed with water in an oligotrophic culture medium to proliferate the Schistostega osmundacea protonemata grown from the gametophore. The method for culturing the Schistostega osmundacea may comprise performing the culture in the culture medium including a stationary oligotrophic agar culture medium, cutting the obtained Schistostega osmundacea protonema, adding the cut Schistostega osmundacea protonemata to another oligotrophic inorganic salt liquid culture medium and then proliferating the protonema under the irradiation with light at 15 to 22°C.;COPYRIGHT: (C)2007,JPO&INPIT