single protein production in living cells messenger RNA interference enzyme to promote

摘要

The single protein production where this invention, the mRNA interference enzyme, for example disassembling all cells mRNA efficiently and selectively with in vivo, utilized the unique quality of MazF of the cell toxin which is one chain RNA and the ACA unique ended ribo- nuclease which slump all the protein syntheses, in the colon fungicolous cell (SPP) the system is stated. In order the cord/code to do MazF, and nothing ACAmRNA, substantially without synthesis of the background cell protein, continuance and high level (90% ones) target revelation occurs with the simultaneous revelation of the target gene which was operated. In it should observing, as for target synthesis at least the continuation and the cell 4 days in spite even in the multiplication stop keeping copying and translation ability are shown. SPP technology, the yeast and the human protein, furthermore is effective to also the bacterial indwelling characteristic membrane protein. It is difficult to handle this new system, so far, but the signal/noise ratio which biologically can simplify the structure of the important protein and the research of function dramatically and is not applied is made possible.