Synthetic method of the labeling reagent and the said reagent which possess the pyridine nucleus which has the diazo methyl functional group and method of detection of the organism molecule

摘要

Formula (C) or (D)[In formula,As for R₁, displaying especially detection possible sign,R₂ and R₃ is independence mutually, H and NO₂, Cl, Br and F, I, OR, SR and NR², R, NHCOR, CONHR or COOR are displayed, here, R is alkyl or aryl,As for R₄, H, displaying alkyl group or aryl basis,L is the linker arm]The labeling reagent which is displayed by. In addition as for this invention, the sign whose it is possible to obtain the sign which is obtained by the labeling method and the aforementioned method of synthesizing and the organism molecule the labeling reagent the organism molecule which is done, with the method and the aforementioned method of the one chain or labeling and fragmentation of two these chain nuclear acids the nuclear acid which is done, the kit in order to detect the target nuclear acid which contains the nuclear acid which the sign is done, it regards the solid backing and the method the reagent is connected in order where to capture nuclear acid.This invention is applied to diagnostic field desirably. Selective figure It is not