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Inclusion body purification by cross-flow microfiltration
Inclusion body purification by cross-flow microfiltration
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机译:通过错流微滤纯化包涵体
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摘要
Purification and/or concentration of protein inclusion bodies (I) in a solution involves passing the solution tangentially across at least one semipermeable membrane (SPM), so that (I) are retained by the SPM and components with lower molecular weight or particle diameter permeate the SPM. Purification and/or concentration of protein inclusion bodies (I) in a solution involves passing the solution tangentially across at least one semipermeable membrane (SPM), so that (I) are retained by the SPM and components with lower molecular weight or particle diameter permeate the SPM. The SPM is formed from intersecting longitudinal and transverse fibers, each having a thickness of 150-600 mu m and a spacing of 5-15 times the thickness.
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