PURIFICATION OF INCLUSION BODIES BY CROSSFLOW MICROFILTRATION

摘要

The invention relates to a method for the purification and/or theconcentration of protein inclusion bodies in a highly concentrated, particle-containing solution. The suspension containing the protein inclusion bodies istangentially passed over one or a plurality of semi-permeable membranes, suchthat the protein inclusion body is retained behind the membranes andsubstances with a lower molecular weight pass through the membrane and/or areadsorbed on the membrane, resulting in a purified and/or concentrated proteininclusion body suspension. In a similar manner cell wall particles may beseparated from a protein inclusion body suspension. The invention furtherrelates to the use of a crossflow microfiltration unit for the purificationand/or concentration of protein inclusion bodies in a suspension and theseparation of cell wall particles from a protein inclusion body preparation.