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Method for transporting potassium ions from front side to back side of lipid bilayer membrane
Method for transporting potassium ions from front side to back side of lipid bilayer membrane
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机译:从脂质双层膜的正面向背面输送钾离子的方法
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摘要
An object of the present invention is to detect a specific chemical substance with high sensitivity and high precision. By using a specific lipid bilayer membrane, a chemical substance is detected with high sensitivity and high precision. Here, the specific lipid bilayer membrane comprises a chemical substance receptor, a chimeric G protein, and a potassium ion channel. The chimeric G protein comprises a chimeric Gα subunit and a Gβγ subunit complex. The chimeric Gα subunit is selected from the group consisting of Gi/olf13 (SEQ ID NO: 04), Gi/olf28 (SEQ ID NO: 05), Gi/olf94 (SEQ ID NO: 07), Gi/olf113 (SEQ ID NO: 08), Gi/olf α3-β5,C (SEQ ID NO: 12), and Gi/olfα4-β6,C (SEQ ID NO: 15).
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机译:本发明的目的是以高灵敏度和高精度检测特定化学物质。通过使用特定的脂质双层膜,可以高灵敏度且高精度地检测化学物质。在此,特定的脂质双层膜包含化学物质受体,嵌合G蛋白和钾离子通道。嵌合G蛋白包含嵌合G α Sub>亚基和G βγ Sub>亚基复合物。嵌合的G α Sub>亚基选自G i / olf13 Sub>(SEQ ID NO:04),G i / olf28 Sub>( SEQ ID NO:05),G i / olf94 Sub>(SEQ ID NO:07),G i / olf113 Sub>(SEQ ID NO:08),G i / olfα3-β5,C Sub>(SEQ ID NO:12)和G i /olfα4-β6,C Sub>(SEQ ID NO:15)。
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