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Method of measuring the ability of a sample to withstand reactive oxygen species (ROSS)

机译:测量样品抵抗活性氧的能力的方法(ROSS)

摘要

A method of measuring the ability of a biological sample to withstand reactive oxygen species (ROSs). The method includes at least the steps of putting the sample for testing and a photosensitive agent in a liquid medium into contact so as to form a mixture for testing, subjecting the mixture for testing to a dose of light irradiation so as to give rise, by photochemical reaction, to the production of reactive oxygen species, then after irradiation, adding a compound that reacts colorimetrically in the presence of reactive oxygen species (ROSs) to form a chromogen or fluorescent substance, and measuring the quantity of chromogen or fluorescent substance that is produced, and also subjecting a reference mixture to the same photochemical, colorimetric, and measurement reactions as the mixture for testing.
机译:一种测量生物样品抵抗活性氧(ROSs)的能力的方法。该方法至少包括以下步骤:通过以下方法使待测样品和液体介质中的光敏剂接触,以形成待测混合物,对待测混合物进行一定剂量的光辐照,从而产生光化学反应,产生活性氧,然后在辐射后,添加一种在活性氧(ROSs)存在下进行比色反应的化合物,以形成色原或荧光物质,并测量色原或荧光物质的量。制得的混合物,并使参比混合物经受与待测混合物相同的光化学,比色和测量反应。

著录项

  • 公开/公告号US8143017B2

    专利类型

  • 公开/公告日2012-03-27

    原文格式PDF

  • 申请/专利权人 THIERRY PATRICE;

    申请/专利号US20080741455

  • 发明设计人 THIERRY PATRICE;

    申请日2008-11-13

  • 分类号C12Q1/02;C12P21/06;C07K1/00;

  • 国家 US

  • 入库时间 2022-08-21 17:27:32

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