ISOLATED POLYNUCLEOTIDES, NUCLEIC ACID CONSTRUCTS, METHODS AND KITS FOR LOCALIZATION OF RNA AND/OR POLYPEPTIDES WITHIN LIVING CELLS

摘要

An isolated polynucleotide comprising a first nucleic acid sequence which comprises two functionally compatible recognition sites for a site-specific recombination enzyme and a second nucleic acid sequence encoding a protein binding-RNA sequence is provided. Also provided are nucleic acid constructs, methods and kits for localization of an mRNA and/or a polypeptide encoded by a given gene-of-interest within living cells.