CHIMERIC HUMAN GROWTH HORMONE DERIVED FROM PLACENTA AND PITUITARY ISOFORM AND PROCESS FOR OBTAINING SAID CHIMERA

摘要

The present invention relates ot a novel hGH-NV cDNA chimera having SEQ ID NO:3 encoding human growth hormone and the invention also relates to a process for obtaining nopvel HGH_NV cDNA chimera from the cDNA isoforms of human placenata and pituitary RNA, amplifying the cDNA isoforms digesting the cloned cDNA specific primers, clonign the amplified cDNa isoforms, digesting teh cloned cDNa isoforms at specififc restriction endonuclease sites follwed by replaci9ng the digested cDNA isoforms hGH-V fragment specififc regiion by g GH-N fragment to obtain hGH-NC cDNA chimera. Further the nove; hGH-NV cDNA chimera is used as a teplate to obtain expressible construct containimng fragment having SEQ ID NO: 5 to produce mature haman growth hormone.