the gene encoding human glucokinase mutant enzyme encoded by the recombinant vectors and recombinant hosts, pharmaceutical compositions and their uses, and processes for the treatment and prevention of diseases

摘要

the invention relates to a gene encoding a mutant human glucokinase.the gene has the nucleotide sequence selected from the nucleotide sequence listed as seq id no: 2 and the nucleotide sequence in the region of open reading frame (orf) that code for the same amino acid sequence encoded by the orf region of the position 487 1 884) of seq id no: 2, and the remaining region is identical to the non orf region of seq id no: 2.the invention also relates to a human glucokinase mutant encoded by the gene, the recombinant vectors containing the gene, host comprising the vectors, pharmaceutical compositions thereof, and methods of their use for the treatment and prevention diseases with them.human glucokinase mutant encoded by the gene has higher activity than the wild type human glucokinase, and provides a new way to control blood glucose, prevention and treatment of disturbance of carbohydrate metabolism, particularly for preventing and treatment of diabetes.