METHOD FOR CARRYING OUT IN VITRO CULTURES OF ARNICA MONTANA L. SPECIES USED FOR EVALUATING THE BIOSYNTHESIS OF SOME BIOLOGICALLY ACTIVE COMPOUNDS

摘要

The present invention relates to a method for carrying out in vitro culture of Arnica montana L. species by developing the following processes: cultures of differentiated tissues (meristematic tissues) and undifferentiated tissues (callus and cell clusters), in order to carry out an integrated evaluation of the biosynthesis of biologically active compounds. These consist of micropropagation cultures (explant - stem apices obtained by the germination of seeds under aseptic conditions) by using a Murashige and Skoog (1962) (MS) culture medium with 25 g/l saccharose, solidified with 8...8.5% agar and supplemented with 1.0 mg/l BAP, callus cultures (explant - foliar and root fragments sampled from in vitro regenerated plants, immature inflorescences) by using a culture medium MS with 30g/l saccharose solidified with 7.5% agar, in three hormonal balances variants (0.5 mg/l BAP and 1.5 mg/l 2.4D, 0.5mg/l kinetine and 1.5 mg/l2.4D, 1.0 mg/l kinetine and 1.0 mg/l NAA), submerged cultures (inoculum - callus obtained under the above-mentioned conditions), by using a culture medium MS (liquid) with 30 g/l saccharose, without growth regulators.