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test methods for detection of viable mikrobenzellen in a sample

机译:样品中可行的米博克伦策伦的测试方法

摘要

The present invention discloses an assay method for the detection of viable microbial cells in a sample, the assay method comprising the steps of: i) adding an ATP degrading enzyme to a sample suspected of containing viable microbial cells to substantially degrade any extracellular ATP in the sample; ii) adding a phosphate containing compound to the sample to substantially halt action of the ATP degrading enzyme; and iii) subjecting the sample to a detection assay to establish the level of undegraded ATP in the sample to provide an indication of the level of viable microbial cells in the sample.
机译:本发明公开了一种用于检测样品中的活微生物细胞的测定方法,该测定方法包括以下步骤:i)向怀疑含有活微生物细胞的样品中添加ATP降解酶,以实质上降解所述细胞中的任何细胞外ATP。样品; ii)向样品中加入含磷酸盐的化合物,以基本阻止ATP降解酶的作用; iii)对样品进行检测测定以确定样品中未降解的ATP的水平,以提供样品中活微生物细胞水平的指示。

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