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USE OF AN ENDOGENOUS 2-MICRON YEAST PLASMID FOR GENE OVER EXPRESSION
USE OF AN ENDOGENOUS 2-MICRON YEAST PLASMID FOR GENE OVER EXPRESSION
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机译:内源性2-微酵母质粒用于基因过表达
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摘要
Methods and compositions for making stable recombinant yeast 2 µm plasmids are provided. Homologous recombination is performed to clone a nucleic acid of interest into the yeast 2 µm plasmid. Heterologous nucleic acid subsequences are recombined between an FLP and a REP2 gene of the plasmid.
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