USE OF AN ENDOGENOUS 2-MICRON YEAST PLASMID FOR GENE OVER EXPRESSION

摘要

Methods and compositions for making stable recombinant yeast 2 μm plasmids are provided. Homologous recombination is performed to clone a nucleic acid of interest into the yeast 2 μm plasmid. Heterologous nucleic acid subsequences are recombined between an FLP and a REP2 gene of the plasmid.