Secretion, transcription and sporulation genes in Bacillus Clausii

摘要

#$%^&*AU2008216973B220120726.pdf#####Abstract The present invention relates to cells that have been genetically manipulated to have an altered capacity to produce expressed proteins. In particular, the present invention relates to Gram-positive microorganisms having exogenous nucleic acid sequences introduced therein and methods for producing proteins in such host cells, such as members of the genus Bacillus. More specifically, the present invention relates to the expression, production and secretion of a polypeptide of interest and to cells that have been genetically manipulated to have an altered capacity to produce expressed proteins. In particular, the present invention provides for the enhanced expression of a selected polypeptide by a microorganism.1/22 m r wo mf 1 m N 'Zi .l l-4 N~ M Mi O H-W 0 H caEq( 0 H c E-4 w zC4 90 ( a W 4H U2 4 I :V4 F P H 0 W E P4 NU) a:q C' C') I Ix ' H N )4HC w 0 0 ) 4 c)H w H z W 0 2 1-J -1 4- W C-Y-~0 H 4 YH H 4 0 E-4H 0Q .. 9 U)4 U)E-Zx 04 I( 4j E- CY 04 0