METHODS OF DETECTING GENETIC POLYMORPHISMS USING MELTING TEMPERATURE OF PROBE-AMPLICON COMPLEXES

摘要

The present application provides methods and related kits for the detection of a nucleotide difference between a target sequence and standard sequences. The methods include the determination of a melting temperature of a complex between an amplicon of the target sequence and a probe which is substantially identical to one of the standard sequences. The probe comprises a detectable label, linked to its 5' end as well as a quencher, linked to its 3' end. This method can be easily adapted for use with multiple probes and in multiplex PCR.