Polypeptides involved in the expression of resistance to glycopeptide antibiotics

摘要

New polypeptide compsn. (A) comprises at least one protein (I) (or part of it) involved in resistance to glycoprotein antibiotics. (I) are identified by reference to a 10851 base DNA sequence (reproduced together with the amino acid sequence). They are VanH (encoded by bases 6018-6983); VanA (6977-7807); VanX (7816-8621) or VanC (10116-10598). Included is any protein (or part of it) which is (a) recognised by antibodies directed against the above 4 proteins or (b) encoded by a sequence which hybridises with (b1) one of the nucleotide chains identified by bases 6977-7807; 6018-6983; 7816-8621 or by a separate 1347 sequence reproduced in the specification, or (b2) the sequences (V1) or (V2). Also new are (1) purified proteins VanH, VanC, VanX, VanA, VanR (bases 3976-4668) and VanS (4648-5800); (2) nucleic acid encoding any of the preceding proteins (including complementary DNA or corresponding RNA); (3) recombinant sequences contg. this nucleic acid and regulatory elements; (4) recombinant vectors or host cells contg. such nucleic acid; (5) nucleotide probes (DNA or RNA) able to hybridise with (recombinant) nucleic acid; (6) mono- or poly-clonal antibodies (Ab) directed against the proteins.