Streptomyces spp. as efficient expression system for a dd-peptidase/dd-carboxypeptidase involved in glycopeptide antibiotic resistance

摘要

BackgroundVanYn, encoded by the dbv7 gene (also known as vanYn) of the biosynthetic cluster devoted to production, is a novel protein involved in the mechanism of self-resistance in Nonomuraea sp. ATCC 39727. This filamentous actinomycete is an uncommon microorganism, difficult-to-handle but biotechnologically valuable since it produces the glycopeptide antibiotic , which is the precursor of the second-generation dalbavancin in phase III of clinical development. In order to investigate VanYn role in glycopeptide resistance in the producer actinomycete an appropriate host-vector expression system is required.