RECOMBINANT MICROORGANISM HAVING AN IMPROVED ABILITY TO PRODUCE BUTANOL AND A PRODUCTION METHOD FOR BUTANOL USING THE SAME

摘要

The present invention relates to a recombinant microorganism having an improved ability to produce butanol and to a production method for butanol using the same, and more specifically relates to a recombinant microorganism for biosynthesis of butanol in which 2-ketoisovalerate, which is a precursor of L-valine, is used as an intermediate, and relates to a production method for butanol using the same. A feature of the recombinant microorganism according to the present invention is that a microorganism having the ability to produce 2-ketoisovalerate, which is a precursor of L-valine, has introduced therein: a gene coding for VorABC enzyme which is an enzyme that converts 2-ketoisovalerate into isobutyryl-CoA; a gene coding for an enzyme that converts isobutyryl-CoA into butyryl-CoA; a gene coding for an enzyme that converts butyryl-CoA into butyraldehyde; and a gene coding for an enzyme that converts butyraldehyde into butanol. Unlike existing Clostridium acetobutylicum, the recombinant microorganism according to the present invention is easy to grow, has a substantial strain-improvement potential due to additional metabolic flux manipulation and is useful as a microorganism for the industrial production of butanol, and a recombinant microorganism is provided which has an improved ability to produce butanol by simplifying the process for converting 2-ketoisovalerate to isobutyryl-CoA.