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METHOD AND KIT FOR THE DETECTION OF HEPARIN-DEPENDENT ANTIBODIES AND THE DIAGNOSIS OF IMMUNE OR AUTOIMMUNE PATHOLOGIES POTENTIATED BY HEPARIN SUCH AS HEPARIN-INDUCED THROMBOCYTOPENIA
METHOD AND KIT FOR THE DETECTION OF HEPARIN-DEPENDENT ANTIBODIES AND THE DIAGNOSIS OF IMMUNE OR AUTOIMMUNE PATHOLOGIES POTENTIATED BY HEPARIN SUCH AS HEPARIN-INDUCED THROMBOCYTOPENIA
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机译:检测肝素依赖性抗体和诊断肝素引起的血小板减少症的免疫或自身免疫病的方法和试剂盒
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摘要
The invention relates to a method for the detection of heparin-dependent antibodies and the diagnosis of immune or autoimmune pathologies potentiated by a heparin substance, such as thrombocytopenia induced by heparin (HIT type II) as inducer drug. The invention is characterized in that said method comprises the steps consisting in: 1) reacting: at least one substance with high affinity for a heparin substance (SFA), consisting of any molecule or complex having a high affinity for a heparin substance, with at least one heparin substance (SH) so as to form a substance with high affinity for heparin-heparin substance (SFA-SH) complex, wherein the heparin substance is in slight or large excess, so as to be able to bind other substances with affinity for heparin; then with at least one potential antigenic substance capable of reacting with the heparin substance, thus forming a complex (Ag-SH); and, finally, with the plasma or the serum of a patient to be tested (preferably diluted to a dilution of the order of 1:100) potentially containing: an anti(Ag-SH) antibody material generated in the organism after administration of a heparin substance, and/or at least one immune complex consisting of an Ag-SH/anti(Ag-SH) antibody complex present in the plasma or serum of the patient capable of reacting with the substance with high affinity for the heparin substance-heparin substance (SFA-SH) complex, and then, 2) revealing the resulting (Ag-SH)-anti(Ag-SH) complex produced.
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