METHOD FOR MULTIPLEX DETECTION OF GENOMIC DNA POLYMORPHISM AND PROBES DEDICATED THERETO

摘要

Provided are a method for multiplex detection of genomic DNA polymorphism and probes dedicated thereto. Also disclosed is a single-stranded DNA molecule comprising successively, from the 5' end to the 3' end, a left-side oligonucleotide fragment complementary to the 5' end of the DNA to be detected, a main fragment and a right-side oligonucleotide fragment complementary to the 3' end of the DNA to be detected. Through ligase reaction, different labels and different allelic sites are distinguished by the single-stranged DNA molecule. Hand-clasping probes of different lengths are successfully ligated by PCR amplification. Through gel or capillary electrophoresis separation and detection systems, simultaneous detection of the polymorphism of a multiple of deoxyribonucleic acids is achieved. High sensitivity and accuracy are attained, and the requirements for high-throughput and low cost are satisfied at the same time.