RECOMBINANT ENDOSPORE WITH HUMAN SERUM ALBUMIN PRESENTED ON SURFACE FOR ORAL ADMINISTRATION AND PREPARATION METHOD THEREFOR

摘要

A method for preparing Bacillus subtilis endospore with human serum albumin fusion protein presented on the surface and an oral product thereof. Capsid protein gene cotC of Bacillus subtilis endospore, as a molecular vector, is recombined with human serum albumin gene hsa, to construct an integrated recombinant plasmid that is expressed as a fusion, the recombinant plasmid that expresses human serum albumin HSA as a fusion is transformed into Bacillus subtilis, an exogenous gene is integrated to an amylase gene amyE at a specific site on the chromosome through homologous recombination, the recombinant strain has no amylase activity due to insertional inactivation of the amylase gene, a recombinant Bacillus subtilis strain that expresses CotC-HAS as a fusion is screened, and the recombinant strain is induced to produce endospore with human serum albumin presented on the surface. The endospore produced by the recombinant strain can be directly orally administered to animals.