PAM-pCD163 CELL LINE FOR ISOLATION OF PORCIEN REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS AND A VACCINE USING THE SAME

摘要

PURPOSE: A PAM-pCD163 cell line for isolating porcine reproductive and respiratory syndrome(PRRS) virus is provided to develop a vaccine and to prevent PRRS.;CONSTITUTION: A PAM-pCD163 cell line is prepared by expressing pCD163 in PAM cells through a retrovirus prepared by transforming plasmid containing pCD163 genes into HEK293 cells. The plasmid is pFB-Zeo-pCD163. A method for preparing the cell line comprises: a step of performing transfection of VSV-G glycoprotein and MMLVgag/pol gene, and a plasmid containing pCD163 into HEK293 cells to prepare retrovirus; and a step of inoculating the retrovirus into the PAM cells.;COPYRIGHT KIPO 2012