THE SIMULTANEOUS DETECTION METHOD OF PATHOGENIC VIBRIO SPP. USING MULTIPLEX REAL-TIME PCR

摘要

PURPOSE: A method for simultaneously detecting three kinds of vibrio bacteria causing food poisoning is provided to quickly detect the presence of the vibrio. CONSTITUTION: A method for simultaneously detecting three kinds of vibrio bacteria, Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus comprises: a step of performing real time PCR of zot, vmrA, and vuuA genes; and a step of detecting species by melting temperature of PCR products. A complementary primer set encoding the zot, vmrA, and vuuA genes is a sequence set 1,2-3,4-5,6 combination.