METHOD FOR PRODUCING A LARGE AMOUNT OF HUMAN COAGULATION FACTOR VII AND A CELL LINE PRODUCING A LARGE AMOUNT OF FACTOR VII

摘要

PURPOSE: A method for producing a large amount of human coagulation factor VII is provided to manufacture a therapeutic agent for treating hemophilia.;CONSTITUTION: A method for producing a large amount of human coagulation factor VII comprises: a step of preparing an expression vector containing a base sequence encoding dehydrofolate reductase and a base sequence of CCGCCC-deleted dehydrofolate reductase promoter; a step of transforming animal cell line with the expression vector; a step of culturing the transformed cell line to selecting a cell line expressing human factor VII; and a step of adding sodium butylate to the animal cell.;COPYRIGHT KIPO 2012