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MEDIUM FOR MAINTAINING AND DETECTING WEST NILE VIRUS AND A POTENCY ASSAY METHOD USING THE SAME
MEDIUM FOR MAINTAINING AND DETECTING WEST NILE VIRUS AND A POTENCY ASSAY METHOD USING THE SAME
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机译:维护和检测西尼罗河病毒的媒介和使用该媒介的潜力评估方法
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PURPOSE: A medium for maintainig and determining west nile is provided to reduce the exposure by high risk virus and to shorten the use of harmful reagents.;CONSTITUTION: A medium for maintaining and detecting west nile contains alpha-MEM or DMEM containing 1-10 g/L of glucose and pH indicator. The pH indicator is phenol red. A potency assay method for detecting west nile comprises: a step of culturing west nile host cells and a sample in the medium; and a step of measuring the pH concentration of the medium to detecting west nile virus and tittering west nile in a sample. The host cells are monkey kidney cells.;COPYRIGHT KIPO 2012
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机译:目的:提供一种用于维护和检测西尼罗河的介质,以减少高风险病毒的暴露并缩短有害试剂的使用。;组成:用于维护和检测西尼罗河的介质包含alpha-MEM或DMEM,其含量为1-10 g / L葡萄糖和pH指示剂。 pH指示剂是酚红。一种检测西尼罗河的效力测定方法,包括:在培养基中培养西尼罗河宿主细胞和样品的步骤;测量培养基pH值以检测西尼罗河病毒和滴定样品中西尼罗河的步骤。宿主细胞是猴肾细胞。; COPYRIGHT KIPO 2012
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