METHOD FOR MANUFACTURING A BIO-HYBRID METAL NANOPROBE FOR MEASURING SURFACE-ENHANCED RAMAN SCATTERING IMAGES

摘要

PURPOSE: A method for manufacturing a bio-hybrid metal nanoprobe for measuring surface-enhanced Raman scattering images is provided to manufacture a bio-hybrid metal nanoprobe for measuring surface-enhanced Raman scattering S images in which sensitivity and chemical stability are high, thereby using the metal nanoprobe as a surface-enhanced Raman scattering based bio imaging device.;CONSTITUTION: A method for manufacturing a bio-hybrid metal nanoprobe for measuring surface-enhanced Raman scattering images comprises next steps. Seed metal nano particles are prepared. The seed metal nano particles and a Raman dye are heated or collected after mixing so that a metal nano-cluster including the seed metal nano particles and Raman dye is formed. The metal nano-cluster is coated with a protein or first hydrophilic polymer so that the metal nano-cluster is formed. The metal nano-cluster coated with the protein or first hydrophilic polymer is mixed with a second polymer solution so that a second polymer suspension including the metal nano-cluster is manufactured. The suspension is sprayed by an electricity hydraulic power spraying method so that the metal nano-cluster encapsulated by covering the nano-cluster with the second polymer. The encapsulated metal nano-cluster is heated or UV treated and a cross-linkage or non-cross-linkage is generated in between the second polymers covering the metal nano-cluster so that a metal nano-cluster-second polymer network is formed.;COPYRIGHT KIPO 2013;[Reference numerals] (AA) On-seed(6 to 13nm); (BB) Raman dye+Na3 - citrate+AgNO3; (CC) Stabilizing with protein; (DD) Raman dye+sa (EE) Stabilizing with polymer; (FF) Silver nano particle plaster(60 to 150nm); (GG) Silver nano cluster(30 to 40nm)