Real-time screening method of protease or its inhibitors activity using unmodified gold nanoparticles

摘要

PURPOSE: A method for real time measurement of hydrolase or inhibitor thereof using stabilized gold nanoparticles is provided to enable screening hydrolase inhibitor with high efficiency. CONSTITUTION: A method for real time measurement of enzyme activity using gold nanoparticles comprises: a step of preparing a mixture solution containing stabilized gold nanoparticles and enzyme reactive substrate; and a step of adding hydrolase to the mixture solution to measure color change. The gold nanoparticles are stabilized by nucleotides, DNA, detergent or polymers. The hydrolase is protease, galatosidase or phophatase. A method for measuring enzyme inhibitor activity using gold nanoparticles comprises: a step of preparing a mixture solution containing stabilized gold nanoparticles, enzyme reactive substrate and enzyme inhibitor; a step of adding a hydrolase; and a step of measuring color change.