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REAL-TIME SCREENING METHOD OF PROTEASE OR ITS INHIBITORS ACTIVITY USING UNMODIFIED GOLD NANOPARTICLES
REAL-TIME SCREENING METHOD OF PROTEASE OR ITS INHIBITORS ACTIVITY USING UNMODIFIED GOLD NANOPARTICLES
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机译:使用未修饰的金纳米粒子的蛋白酶或其抑制剂活性的实时筛选方法
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摘要
PURPOSE: A method for real time measurement of hydrolase or inhibitor thereof using stabilized gold nanoparticles is provided to enable screening hydrolase inhibitor with high efficiency. CONSTITUTION: A method for real time measurement of enzyme activity using gold nanoparticles comprises: a step of preparing a mixture solution containing stabilized gold nanoparticles and enzyme reactive substrate; and a step of adding hydrolase to the mixture solution to measure color change. The gold nanoparticles are stabilized by nucleotides, DNA, detergent or polymers. The hydrolase is protease, galatosidase or phophatase. A method for measuring enzyme inhibitor activity using gold nanoparticles comprises: a step of preparing a mixture solution containing stabilized gold nanoparticles, enzyme reactive substrate and enzyme inhibitor; a step of adding a hydrolase; and a step of measuring color change.
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