si RNA-Hydrophilic Polymer Conjugates for gene delivery to plant cells and Method for gene delivery to plant cells using the same

摘要

The present invention relates to a gene transfer method into siRNA (short interference RNA) with a hydrophilic polymer conjugates and plant cells using the above junction body can function as a gene delivery system of the plant cells, is more particularly Hasegawa PEI, PVP, PEG or PVA and the siRNA is directed to a method for increasing the gene transfer efficiency through a, a joined body and an ultrasonic treatment to ensure stability for the gene transfer into the plant cells combined in a specific ratio. ; Since si RNA and hydrophilic polymer conjugate of the present invention can deliver the gene to the plant cell to the cell wall is present, parallel processing of the ultrasonic wave is to maximize gene transfer efficiency by promoting the plant cell permeability, according to the present invention, a plant cell is able to determine the function of a specific gene in a low-cost high efficiency through a control expression of a specific gene in the plant cell. ; Plant cells, si RNA, the hydrophilic polymer, PVP, PVA, gene delivery, ultrasonic