METHODS FOR RIPPING OVARIAN FOLICULES IN VITRO

摘要

1. A method of in vitro maturation of ovarian follicles and oocytes, comprising the use of PTEN inhibitors. ! 2. The method of claim 1, wherein the ovarian follicles are non-growing follicles, such as primordial, intermediate, and primary follicles. ! 3. The method of claim 1, wherein the mature oocytes are additionally used for in vitro fertilization. ! 4. The method of claim 1, wherein the ovarian follicles or oocytes are human. ! 5. The method of claim 1, wherein the ovarian follicles or oocytes are obtained from an animal. ! 6. The method according to any one of paragraphs. 1-5, where the PTEN inhibitor is selected from:! Bisperoxo (bipyridine) oxovanadate,! Bisperoxo (l, 10-phenanthroline) oxovanadate,! Bisperoxo (picolinato) oxovanadate,! Bisperoxo (5-hydroxypyridin-2-carboxyl) oxovanadate,! Di- (picolinate) oxovanadate,! Di- (3-hydroxypicolinate) oxovanadate,! Bisperoxo (phenylbiguanide) oxovanadate,! Di- (phenylbiguanide) oxovanadate and! Bisperoxo (isoquinoline carboxylic acid) oxovanadate. ! 7. A method of in vitro maturation of an oocyte, which includes activation of the follicles according to claim 1.! 8. An in vitro fertilization method, including implanting an embryo in need thereof, wherein said embryo is obtained by a method comprising treating a mature oocyte with seminal fluid, wherein said oocyte is obtained by the method of claim 7.! 9. A method for identifying compounds that can potentially be used to mature in vitro ovarian follicles and / or oocytes, the method comprising measuring the ability of the compound to act as a PTEN inhibitor. ! 10. The use of one or more PTEN inhibitors to obtain a medicinal product for use in improving