METHOD FOR ISOLATION AND CLEANING OF NUCLEIC ACIDS FROM LIQUID MEDIA (OPTIONS) AND VESSELS FROM PLASTIC FOR SORPING NUCLEIC ACIDS FROM LIQUID MEDIA

摘要

1. A vessel made of plastic for sorption of nucleic acids from a liquid medium, comprising a coating of silicon oxide on the inner surface of the vessel, characterized in that the coating is a nanocoating made by thin-film synthesis. ! 2. The vessel according to claim 1, characterized in that the plastic is selected from the group consisting of polyvinyl chloride, polyethylene, polypropylene, polystyrene, polycarbonates, acrylonitrile butadiene styrene, polyurethanes, polyamides, thermoplastic elastomers, polysulfones and polyetheretherketones, as well as mixtures thereof. ! 3. The vessel according to claim 1, characterized in that the vessel is made optically transparent. ! 4. The vessel according to claim 1, characterized in that it is made in the form of a test tube. ! 5. The vessel according to claim 1, characterized in that said thin-film synthesis is realized at ultrahigh vacuum. ! 6. The vessel according to claim 5, characterized in that said thin-film synthesis comprises ion-plasma spraying. ! 7. The vessel according to claim 6, characterized in that the ion-plasma spraying is performed by sputtering a quartz target with a stream of Ar + ions in vacuum. ! 8. The vessel according to claim 5, characterized in that the inner surface of the vessel before spraying is chemically cleaned with alcohol in an ultrasonic bath. ! 9. The vessel according to claim 1, characterized in that the thickness of the nanocoating does not exceed 25 nm. ! 10. A method of isolating and purifying nucleic acids from a liquid medium, including sorption on the inner walls of the vessel, washing from impurities and elution in saline, characterized in that silicon oxide nanocoating made by thin-film synthesis is used as a sorbent. ! 11. The method according to claim 10, characterized in that the use of silicon oxide nanocoating with sizes from 1 to 25 nm. ! 12. C