METHOD FOR DIFFERENTIATING MAMMAL PRECEDENT CELLS IN INSULIN-PRODUCING ISLAND CELL CELLS

摘要

1. A method for differentiating progenitor cells into insulin-producing pancreatic cells, comprising:! (a) incubation of the progenitor cells in serum-free pre-induction media containing sodium butyrate; and then ! (b) incubation of the progenitor cell in induction media containing knockout serum substitute! for a period of time sufficient to differentiate the progenitor cells into insulin-producing pancreatic cells. ! 2. The method of claim 1, wherein the progenitor cells are human progenitor cells. ! 3. The method of claim 1, wherein the progenitor cells are liver stem cells. ! 4. The method of claim 1, wherein the progenitor cells are stem cells of the connective tissue of the umbilical cord. ! 5. The method according to claim 1, in which the precursor cells are stem cells of adipose tissue. ! 6. The method according to claim 1, in which the induction media contain about 2% knockout serum substitute. ! 7. A method for differentiating progenitor cells into insulin-producing pancreatic cells, containing! (a) incubation of the progenitor cells in a serum-free pre-induction medium containing a histone deacetylase inhibitor (HDACI) and then! (b) incubation of the progenitor cell in induction media containing a knockout serum substitute,! for a period of time sufficient to differentiate the progenitor cells into insulin-producing pancreatic cells. ! 8. The method according to claim 7, in which HDACI is sodium butyrate, phenylbutyrate, retinoic acid, valproic acid, “APHA” compound 8 (APHA Compound 8), apicidine, () depudec