AIM: To observe the plasticity of whether dermis-derived multipotent cells to differentiate into insulin-producing pancreatic cells in vitro.METHODS: A donal population of dermis-derived multipotent stem cells (DMCs) from newborn rat with the capacity to produce osteocytes, chondrocytes, adipocytes and neurons was used. The gene expression of cultured DMCs was assessed by DNA microarray using rat RGU34A gene expression probe arrays. DMCs were further cultured in the presence of insulin complex components (Insulintransferrin-selenium, ITS) to observe whether DMCs could be induced into insulin-producing pancreatic cells in vitro.RESULTS: DNA microarray analysis showed that cultured DMCs simultaneously expressed several genes associated with pancreatic cell, neural cell, epithelial cell and hepatocyte,widening its transcriptomic repertoire. When cultured in the specific induction medium containing ITS for pancreatic cells, DMCs differentiated into epithelioid cells that were positive for insulin detected by immunohistochemistry.CONCLUSION: Our data indicate that dermal multipotent cells may serve as a source of stem/progenitor cells for insulin-producing pancreatic cells.
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机译:Fucoidan ameliorates pancreatic 尾鈥恈ell death and impaired insulin synthesis in streptozotocin鈥恡reated 尾 cells and mice via a Sirt鈥?鈥恉ependent manner
机译:Fucoidan ameliorates pancreatic 尾鈥恈ell death and impaired insulin synthesis in streptozotocin鈥恡reated 尾 cells and mice via a Sirt鈥?鈥恉ependent manner
机译:Real-time Simultaneous Measurement of Pancreatic β Cell Electrophysiology and Fluorescent Bioimaging Based on High-resolution Thin-film Transistor Microelectrode Arrays
机译:Retraction Notice: Jinshui He Xu Zhang Chaowei Lian Jinzhi Wu Yanling Fang Xiaoling Ye. Exendin-4 prevented pancreatic beta cells from apoptosis in (Type I) diabetic mouse via keap1-Nrf2 signaling