Isolation of Insoluble Enzyme from Enzyme Bouillon and Preparation of Insoluble Enzyme

摘要

1. A method of isolating an insoluble enzyme from a microbial broth containing microbial cells and / or cell debris obtained from microbial cells, comprising isolating an insoluble enzyme from a microbial broth without removing microbial cells and / or cell debris, thus leading to the formation of a composition, containing the selected insoluble enzyme and microbial cells and / or cell debris, in which at least a portion of the enzyme is insoluble in the microbial broth. ! 2. The method according to claim 1, wherein said microbial broth is obtained by a method selected from:! preparing a mixture of the enzyme with the microbial cell containing and / or cell debris, the microbial broth in which the microbial cells do not produce the enzyme and in which the enzyme is insoluble or soluble, and in which case the method further comprises converting at least a portion of the enzyme to an insoluble form by adding a precipitating agent ; ! adding a precipitating agent to the microbial broth containing the soluble enzyme expressed by the microbial cells, thereby causing at least a portion of the enzyme to become insoluble; and! expression of microbial cells of the enzyme in microbial broth, in which at least a portion of the enzyme is insoluble. ! 3. The method according to claim 1, wherein said isolation process comprises at least one isolation procedure selected from preparing the broth; cell lysis; homogenization; diafiltration; and separation of solid and liquid phases! in which the specified selection process includes the execution of two or more of these selection procedures, �