BETA-GALACTOSIDASE WITH TRANSGALACTOSYLATION ACTIVITY

摘要

FIELD: medicine.;SUBSTANCE: what is offered is a DNA molecule recovered from Bifidobacterium bifidum, with a sequence presented in the description, and its degenerate derivative coding β-galactosidase. Also, what is presented is a p-galactosidase enzyme having a sequence presented in the description. What is offered is an expression vector containing said DNA, and a bacterial host cell containing said vector. What is offered is application of the enzyme or the host cell for preparing a mixture of oligosaccharides which can be used for preparation of foodstuff, forages for animals and medicines. What is described is a method for producing the enzyme involving cultivation of said host cell in an appropriate culture medium under conditions supposing expression of said enzyme and recovery of the prepared enzyme from the culture.;EFFECT: invention allows transforming lactose into a mixture of oligosaccharides.;12 cl, 5 dwg, 3 tbl, 2 ex