NOVEL VIRULENCE DETERMINANT WITHIN E2 STRUCTURAL GLYCOPROTEIN OF CLASSICAL SWINE FEVER VIRUS

摘要

FIELD: chemistry.;SUBSTANCE: invention relates to biotechnology, virology and veterinary. An attenuated recombinant classical swine fever virus (CSFV) is described. Modification is carried out by progressively mutating a portion of the E2 gene of the highly pathogenic strain Brescia. As a result, two to six amino acids on the section 829-837 are replaced with two to six amino acids which are characteristic for the heterologous E2 glycoprotein of the Bovine Viral Diarrhea Virus (BVDV). A classical swine fever vaccine is also obtained based on the obtained attenuated CSFV. The invention can be used in veterinary.;EFFECT: classical swine fever vaccine is obtained.;7 cl, 7 dwg, 4 tbl, 7 ex