METHOD OF SURGICAL SIMULATION OF OXIDATIVE STRESS IN LABORATORY ANIMALS

摘要

FIELD: medicine.;SUBSTANCE: simulation procedure is enabled by creating a acute and chronic body injury. After a surgical site is processed with an alcohol solution, an acute process - soft tissue abscess is invoked under regional anaesthesia with 0.5% Novocaine 20-200 ml. For this purpose skin is incised with subcutaneous fat, superficial and thoracolumbar fascias, a fascia of erector muscle of spine are dissected. A sterile gauze sponge impregnated in a daily culture of a pathogenic bacterial strain of the concentration of 10³/ml-10⁸/ml or impregnated in the liquid 1 ml prepared by dissolving animal's excrements 1 gram in 0.9% sodium chloride 1-10 ml. Then the wound is closed by a purse-string suture applied on skin edges. Thereafter within 72-120 hours after the implantation of the infected foreign body, the process is transformed into the chronic form by creating a septic wound. That is ensured by removing cutaneous sutures, extracting the foreign body and performing abscess cavity sanitation.;EFFECT: method provides the severity and length-controlled, two-phase experimental model of the oxidative stress enabling the high-efficiency induction of a required imbalance of the pro-oxidant and antioxidant systems, and having a recovery progression.;3 dwg, 1 ex