HYBRID CELL STRAIN OF MUS MUSCULUS 13F8 ANIMALS - PRODUCER OF MONOCLONAL ANTIBODIES SPECIFIC TO CAPSULAR F1 ANTIGEN YERSINIA PESTIS

摘要

FIELD: medicine.;SUBSTANCE: hybrid cultured cell strain of the animals Mus museums 13F8 is produced by immunisation of Balb/c mice. The mice are immunised by four introductions of the recombinant antigen preparation F1 100 mcg/mouse. The third post-immunisation day is followed by splenocyte hybridisation of the immunised mice (1x10⁸ cells) and mice myeloma cells P3-X63 Ag/8-653 (1×10⁷cells). Polyethylene glycol (Sigma, the USA) is used as a fusion agent. The hybridisation is followed by hybridoma selection, screening, cloning and cryopreservation. Hybridoma is deposited in the State Collection of Pathogens and Cell Cultures of GKPM-Obolensk, No. N-18.;EFFECT: hybrid cultured cell strain producing monoclonal antibodies is applicable for constructing the based plague agent test systems.;7 dwg, 4 tbl, 8 ex