STRAIN OF HYBRID ANIMAL CELLS Mus musculus 5G6 - PRODUCER OF MONOCLONAL ANTIBODIES SPECIFIC TO Yersinia pestis V ANTIGEN

摘要

FIELD: chemistry.;SUBSTANCE: invention relates to biotechnology and can be used to obtain monoclonal antibodies against the Yersinia pestis V antigen. The strain of hybrid animal cells Mus musculus 5G6 is obtained by immunising BALB/c mice. The mice are immunised by four-time administration of a recombinant V antigen in a dose of 100 mcg/mouse. On the third day after the last immunication, splenocytes of immune mice (1×10⁸ cells) are hybridised with mouse myeloma cells RZ-X63 Ag/8-653 (1×10⁷ cells). The fusion agent used is polyethylene glycol (Sigma, USA). Hybridisation is followed by selection, screening, cloning and cryopreservation of the hybridoma. The strain is deposited in the state collection of pathogenic microorganisms and cell cultures (GKPM-Obolensk) under number N-19.;EFFECT: strain of hybrid cultured cells, which produces monoclonal antibodies which are specific to the Y pestis V antigen, is suitable for constructing test systems for detecting plague pathogens.;8 dwg, 2 tbl, 7 ex