METHOD FOR DETECTION OF NEUTROPHIL EXTRACELLULAR TRAPS IN MUCOSAL SECRETIONS

摘要

FIELD: medicine.;SUBSTANCE: method for detection of neutrophil extracellular traps in mucosal secretions provides staining of a native preparation 0.1 ml with an equally component mixture 0.1 ml of the Evans blue dye in the concentration of 1:8000 and the Sytox Green dye in the concentration of 1:500, incubation for 5 minutes in the dark. Luminescent microscopy with using filters generating excitation light at wave length max. 490 nm and emission at wave length 520 nm is used to detect neutrophil extracellular traps, living bacterial cells, apoptotic bacterial cells, green cork bacterial cells. The number of neutrophil extracellular traps (%) containing bacteria in 100 counted traps is evaluated.;EFFECT: method enables visualising chromantin, assessing cell viability and accelerating the study.;2 tbl, 3 ex