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Method for detecting, differentiating and quantifying T-cell populations using reverse transcription quantitative real-time PCR (RT-qPCR) technology
Method for detecting, differentiating and quantifying T-cell populations using reverse transcription quantitative real-time PCR (RT-qPCR) technology
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机译:使用逆转录定量实时PCR(RT-qPCR)技术检测,区分和定量T细胞群体的方法
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摘要
The present invention relates to a method for detecting, differentiating and quantifying T-cell populations comprising the following steps: a) contacting a first aliquot of a body fluid of an individual with at least one antigen, the body fluid comprising antigen presenting cells (APC) and T cells b) incubating the first aliquot with the at least one antigen over a period of time; c) detecting and differentiating the T cell populations by detecting at least a first marker of the APC induced by the T cells of a particular T cell population in the first aliquot and in a second aliquot of the body fluid of the subject not incubated with the at least one antigen, by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR), and d) detecting and quantifying the T-cell populations by determining the ratio of the T cell populations detected marker of APC of the first aliquot to the second aliquot and a kit for carrying out the method.
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