de novo CARCINOGENIC PANCREATIC CANCER CELL STRAIN

摘要

PROBLEM TO BE SOLVED: To provide a pair of a pancreatic cancer model mouse having rapid oncogenicity and cancer cell proliferation potency and a model mouse serving as suitable control for the same, and to simultaneously establish a pancreatic cancer cell strain carcinogenic de novo and a suitable control strain for the same.;SOLUTION: A mating method utilizing a Cre system is used to prepare (a) a human pancreatic cancer model mouse holding "Kras^G12D" and "tsA58Tag" and having rapid oncogenicity and (b) its control model mouse not holding the "Kras^G12D" but holding only the "tsA58Tag", which are born from the same parents formed of a mouse having the "Kras^G12D" and "tsA58Tag" and a mouse holding a Cre gene, and a "rapidly oncogenic pancreatic cancer model cell strain" and a "pancreatic ductal epithelial cell strain" serving as control are established from cells recovered from the mouse pancreas. It is further confirmed that a rapidly oncogenic pancreatic cancer model non-human animal pair can be created by transplanting each of these cell strains. The rapidly oncogenic human pancreatic cancer model mouse pair, the pancreatic cancer model non-human animal pair, and the "rapidly oncogenic pancreatic cancer model cell strain" pair are all excellent as human pancreatic cancer specific analysis tools and effective as a screening system of candidate substances of human pancreatic cancer specific anticancer agents.;COPYRIGHT: (C)2014,JPO&INPIT