The composition and method in order to make the multiplication of the versatile characteristic human trunk cell possible

摘要

The human trunk cell the composition and process in order with in vitro to culture in not yet differentiation state are disclosed. In regard to this point, when it rearranges the human embryo characteristic trunk cell and, it cultures on the plate which with the raminin 10 (raminin 511) was covered, it multiplied, maintained that versatile characteristic. For example, being the composition in order to make the self duplication of the versatile characteristic human trunk cell which grew with in vitro possible, it includes the backing material and the complete rearrangement raminin 511 (LN 511 or raminin 10), the animal protein or the feeder cell are not included, coating, and the feeder cell the known constitution nutrient medium which is not includedIt includes, the composition is offered.