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Agrobacterium strain modified to enhance plant transformation rate

机译:改良农杆菌菌株以提高植物转化率

摘要

Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains. Plants made using these Agrobacterium strains are also described.
机译:提供了在能够独立于农杆菌染色体,Ti质粒和植物转化二元载体进行复制的质粒上带有转化增强基因的农杆菌菌株,并提供了用于这些农杆菌菌株的用途。此外,农杆菌菌株缺乏DNA重组功能,导致植物转化二元载体不稳定或重排,并且在能够独立于农杆菌染色体,Ti质粒和植物转化二元复制的质粒上带有转化增强基因还提供了载体以及这些菌株的用途。还包括在不干扰或以其他方式损害农杆菌细胞的正常生长和植物转化能力的位点处具有整合到农杆菌染色体中的转化增强基因的农杆菌菌株,并且用于这些农杆菌菌株。还描述了使用这些农杆菌菌株制成的植物。

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